西安交通大学学报（医学版）2011，Vol.32Issue(6)：772-777,6.
鸦胆子油脂质体对人肝癌细胞株HepG2抑制作用的体内外研究
Suppression of human hepatocellular cancer cell line HepG2 by Brucea liposome in vitro and in vivo
石磊 1岳媛 2王作仁1
作者信息
- 1. 西安交通大学医学院第一附属医院肝胆外科,陕西西安710061
- 2. 西安交通大学医学院第一附属医院药剂科,陕西西安710061
- 折叠
摘要
Abstract
Objective To study the influence of Brucea liposome on human hepatocellular cancer cell line HepG2 and its mechanisms in vitro and in vivo. Methods Morphologic change of HepG2 cells was observed by transmission electron microscopy after treated with Brucea liposome in vitro; cell proliferation was detected by MTT assay after treated with different doses of Brucea liposome. Flow cytometry was used to detect apoptosis rate at different doses. Nude mice were divided into 4 groups randomly: negative control group, low-dose treatment group, large-dose treatment group and positive control group; the animals were raised in similar conditions for 40 days, had the weight and pathologic changes of the liver recorded at the death point. The apoptotic hepatocarcinoma cells were detected by TUNEL technique. Results Apoptotic cells were found with transmission electron microscope after HepG2 cells were treated with Brucea liposome at 5mg/L for 6 hours in vitro. The proliferation of HepG2 was inhibited significantly by Brucea liposome in a time-dependant manner (when the dose was 2.5 mg/L and 5 mg/L) (P＜0.01), which was stronger than that by Brucea javanica oil emusion ( P＜0.05). Compared with the animals in negative control group, the animal models of treatment groups had a greater weight and a lower metastasis rate at the death point (P＜0. 01). The rate of apoptosis of hepatocellular carcinoma tissues in treatment groups was gradually increased than that in control group (P＜0.01); there were significant differences between the treatment groups (P＜0.01) with TUNEL technique. Conclusion Brucea liposome can inhibit the proliferation of HepG2 in vitro and in vivo, and it can induce the apoptosis of the cancer cells.关键词
鸦胆子油脂质体/肝细胞肝癌/生物学行为/凋亡/荷瘤裸鼠/病理改变/电镜Key words
Brucea liposome/hepatocellular carcinoma/biological behavior/apoptosis/nude mice/pathologic change/transmission electron microscopy (TEM)分类
医药卫生引用本文复制引用
石磊,岳媛,王作仁..鸦胆子油脂质体对人肝癌细胞株HepG2抑制作用的体内外研究[J].西安交通大学学报（医学版）,2011,32(6):772-777,6.
