陕西医学杂志2011，Vol.40Issue(11)：1443-1446,4.
大鼠SOCS3腺病毒的构建及鉴定
Construction and identification adenovirus of SOCS3
摘要
Abstract
Objective:To construct and identify the recombinant adenovirus containing SOCS3 and detect expression in HEK293 cells. Methods, The SOCS3 gene was cloned by RT-PCR after total RNA extracted from peritoneal macrophage of rat, then subcloned with shuttle plasmid pAdtrack-CMV containing GFP reporter gene. pAdeasy-1 adenovirus system was used for co-transfecting shuttle plasmid and frame plasmid to BJ5183 cells. Reconstructed adenovirus was transfected to HEK293 cell after linearization and mRNA and protein level of SOCS3 in the cell were detected. Results; SOCS3 gene was cloned and recombinant adenovirus pAd-SOCS3-GFP was constructed successfully. Recombinant adenovirus was transfected to HEK293 cell and the mRNA and protein level of SOCS3 in the cell were both increased significantly. Conclusions Recombinant SOCS3 adenovirus has been constructed successfully and could be used for transfection in vivo or in vitro.关键词
SOCS3/腺病毒/基因转染/大鼠Key words
@SOCS3/@Adenovirus/@Gene tranfection/Rats分类
医药卫生引用本文复制引用
梁潇,贺明,陈涛,惠智艳,袁祖贻..大鼠SOCS3腺病毒的构建及鉴定[J].陕西医学杂志,2011,40(11):1443-1446,4.基金项目
国家自然科学基金资助项目(No.81025002 （）
No.30971219)陕西省中医管理局资助项目 （）
