中医药学报2011，Vol.39Issue(5)：57-59,3.
寒、热方剂对水浸应激型胃溃疡寒、热证模型大鼠胃组织SOD活性、MDA含量的影响
The Influence of Cold or Heat Prescription on SOD Activity,MDA Contents in Gastric Tissue on Gastric Ulcer Induced by Bondage Water Immersion Stress Combined with Cold or Heat Syndrome Model Rats
摘要
Abstract
Objective: To explore the free radical metabolize mechanism of gastric ulcer model induced by bondage water immersion stress combined with cold or heat syndrome, and the influence of cold or heat prescription on free radical me-tabolizable mechanism. And to observe the basic mechanism of action and objective regularity of "formula - syndrome corresponding" of cold or heat formula on gastric ulcer model combined with cold or heat syndrome. Methods; Adopting cold or heat factors combined with bondage water immersion stress methods to establish gastric ulcer model combined with cold and heat syndrome. Using xanthine oxidase method to detect the activity of SOD and thibabituric acid(TBA) method to detect the contents of MDA in gastric tissue. Results:Compared with the blank group, the activity of SOD decrease remarkably in gastric ulcer model induced by bondage water immersion stress group, gastric ulcer model induced by bondage water immersion stress combined with cold syndrome group ^ gastric ulcer model induced by bondage water immersion stress combined with heat syndrome group (P < 0. 01). But there was no difference between the cold syndrome group and the heat syndrome group. Compared with the blank group, the contents of MDA increase remarkably in gastric ulcer model induced by bondage water immersion stress group, gastric ulcer model induced by bondage water immersion stress combined with heat syndrome group 、gastric heat syndrome group(P <0. 01). Especially the gastric ulcer model induced by bondage water immersion stress combined with heat syndrome group. Dahuang Huanglian Xiexin decoction or Lizhong pill can respectively regulate the free radical metabolize of cold model or heat model. Conclusions: On the basis of the theory of "formula - syndrome corresponding" , verifying the feasibility of duplicating "disease - syndrome combination" model, that is gastric ulcer model combined with cold or heat syndrome. There is the abnormal free radical metabolization in gastric ulcer model induced by bondage water immersion stress combined with cold or heat syndrome. MDA can be regarded as the microcosmic indication of gastric ulcer model induced by bondage water immersion stress combined with heat syndrome.关键词
束缚水浸应激/胃溃疡/寒、热证/SOD/MDAKey words
Bondage water immersion stress/Gastric ulcer/Cold or heat syndrome/SOD/MDA分类
医药卫生引用本文复制引用
李冀,毕珺辉,柴剑波,李胜志,高彦宇,肖洪彬,赵雪莹..寒、热方剂对水浸应激型胃溃疡寒、热证模型大鼠胃组织SOD活性、MDA含量的影响[J].中医药学报,2011,39(5):57-59,3.基金项目
“十一五”国家科技支撑计划项目-“方证相应”模式之复方药理研究(编号:2006BA108B04-03) （编号:2006BA108B04-03）
黑龙江省中医药管理局项目(编号:ZHY10-W01) （编号:ZHY10-W01）
哈尔滨市青年创新人才基金(编号:2009 RFQXS003) （编号:2009 RFQXS003）
