中医学报2011，Vol.26Issue(11)：1338-1340,3.
不同产地清蒸熟地黄饮片高效液相指纹图谱研究
HPLC Fingerprint Chromatogram of Radix Rehmanniae Preparata Steamed in Different Producing Areas
摘要
Abstract
Objective:HPLC fingerprints of Radix Rehmanniae Preparata steamed in different producing areas were studied contrastively to establish the safety and efficacy quality control methods. Methods; RP - HPLC was used and Acteoside was used as the standard. The chromatographic conditions were as follow;column using Polaris C18 (5 μm,4.6 x250 mm) ,the detection wave length was at 334 nm, the flow - rate was at 1.0 mL/min constantly, column temperature was at 25 ℃ , and the test solutions were accurately injected 20 mL respectively into the column. Mobile phase using solvent A and B in linear gradient elution(0 -40 min,A 10% -20% ;40 -60 min,A 20% -30% ) .solvent A and B were acetonitrile and 0. 1% acetic acid solution respectively. Results; HPLC fingerprints of Radix rehmanniae Preparata steamed in different producing areas were greatly similarity,the. Similarity was up to 0. 9. The analysis was carried out by reproducibility of the test( n = 5 ) according to the relative retention times and the relative peak areas of peaks. The RSD of retention times range from 0. 10% to 0. 41% ,and the others range from 0.14% to 1. 70% . Conclusion:The method was specific and the major characteristic fingerprint peaks were obvious, it suitable for quality identification of radix rehmanniae preparata.关键词
熟地黄/高效液相色谱/指纹图谱Key words
Radix Rehmanniae preparata/HPLC/fingerprints分类
医药卫生引用本文复制引用
李军,俞桂新,张丽萍,王晓阁..不同产地清蒸熟地黄饮片高效液相指纹图谱研究[J].中医学报,2011,26(11):1338-1340,3.基金项目
国家科技部“十五”科技攻关重大科研项目(编号:2001 BA701A551-22) （编号:2001 BA701A551-22）
