色谱2011，Vol.29Issue(11)：1049-1054,6.DOI:10.3724/SP.J.1123.2011.01049
基于液相色谱-质谱联用技术的代谢组学方法用于中药通心络和人参对过度疲劳大鼠干预作用的评价
Evaluation of the effect of the traditional Chinese medicine Tongxinluo or ginseng on excess fatigue rats studied by metabonomics approach based on liquid chromatography-mass spectrometry
摘要
Abstract
Excess fatigue is a pathological state of continuing accumulation of fatigue, which may cause the deterioration of body health, occurrence of diseases, and even lead to death. A metabonomics study was performed on the excess fatigue rats treated with traditional Chinese medicine Tongxinluo or ginseng based on ultra fast liquid chromatography coupled with ion trap-time of flight mass spectrometry ( UFLC-IT-TOF-MS ). The plasma metabolic profiling data of the control rats, excess fatigue rats, and excess fatigue rats treated with Tongxinluo or ginseng were acquired. The orthogonal partial least squares analysis ( OPLS ) was applied for the multivariate statistics and the discovery of important differential metabolites distinguishing the excess fatigue rats treated with Tongxinluo or ginseng from the control rats and excess fatigue rats. The results showed tryptophan, bile acid, lysophosphatidylcholine metabolism were disturbed in the excess fatigue rats. The metabolic pattern including the related metabolic pathways of the rats, being treated with Tongxinluo or ginseng, was adjusted towards the normal state.关键词
液相色谱-质谱联用/正交偏最小二乘法/过度疲劳/代谢组学/通心络/人参Key words
liquid chromatography-mass spectrometry ( LC-MS )/ orthogonal partial least squares analysis ( OPLS )/ excess fatigue/ metabonomics/ Tongxinluo/ ginseng分类
化学化工引用本文复制引用
戴伟东,张凤霞,贾振华,魏聪,高鹏,路鑫,吴以岭,许国旺..基于液相色谱-质谱联用技术的代谢组学方法用于中药通心络和人参对过度疲劳大鼠干预作用的评价[J].色谱,2011,29(11):1049-1054,6.基金项目
国际科技合作项目(2009DFA41250)和科技部国家重点基础研究发展计划(2005CB523301,2012CB518606). （2009DFA41250）
