色谱2011，Vol.29Issue(11)：1087-1092,6.DOI:10.3724/SP.J.1123.2011.01087
淀粉及纤维素三(3-三氟甲基苯基氨基甲酸酯)手性固定相的制备及其性能评价
Preparation and evaluation of amylose and cellulose tris(3-trifluoromethylphenylcarbamates)-based chiral stationary phases
摘要
Abstract
To broaden the category of polysaccharide-based chiral stationary phases ( CSPs ), coated CSPs based on tris( 3-trifluoromethylphenylcarbamates ) of amylose and cellulose were prepared for enantioseparation in high performance liquid chromatography. Their performances were evaluated by eight racemates using hexane-isopropanol as mobile phase. Compared with the most widely used, commercially available polysaccharide-based CSPs, Chiralpak AD and Chiralcel OD, utilizing tris( 3,5-dimethylphenylcarbamates ) of amylose and cellulose as the chiral selector, respectively, the obtained CSPs exhibited lower enantioseparation abilities. However, cellulose tris( 3-trifluoromethylphenylcarbamate )-based CSP exhibited characteristic enantioseparation and some chiral compounds were better resolved on this CSP than on Chiral-cel OD. The chiral recognition abilities of the obtained CSPs were increased with the decrease of the portion of isopropanol in the mobile phase and relatively high enantioseparation was obtained with the mobile phase of hexane-isopropanol ( 95: 5, v/v ). The obtained amylosic phase demonstrated slightly higher chiral resolving ability than the cellulosic one for the test racemates. In addition, it was revealed that the enantioseparations of the cellulosic and amy-losic CSPs were complementary.关键词
手性固定相/涂敷/手性识别/多糖衍生物Key words
chiral stationary phase ( CSP )/ coating/ chiral recognition/ polysaccharide deriva-tive分类
化学化工引用本文复制引用
金召磊,胡芳芳,汪一波,刘贵花,王芳,潘富友,唐守万..淀粉及纤维素三(3-三氟甲基苯基氨基甲酸酯)手性固定相的制备及其性能评价[J].色谱,2011,29(11):1087-1092,6.基金项目
国家自然科学基金青年科学基金项目(No.20905057). （No.20905057）
