色谱2011，Vol.29Issue(11)：1103-1106,4.DOI:10.3724/SP.J.1123.2011.01103
超声波萃取-高效液相色谱测定咖啡粉和速溶咖啡中的葫芦巴碱
Determination of trigonelline in coffee powder and instant coffee by ultrasonic extraction and high performance liquid chromatography
摘要
Abstract
A method of high performance liquid chromatography was established for the analy-sis of trigonelline in coffee powder and instant coffee. The separation was performed on a BondPak NH2 column ( 250 mm ×4. 6 mm, 5 μm ). The mobile phase was methanol-water ( 82: 18, v/v) at a flow rate of 0. 8 Ml/min. The detection wavelength was set at 260 nm. The meth-od had good linearity in the range of 1 -40 mg/L (the correlation coefficient was 0. 999 8 ). The repeatability of the method was performed at one day, different days, and by two analysts. The accuracy of the method was evaluated by recovery study. The recoveries were more than 90% with the relative standard deviations less than 3%. The contents of trigonelline in samples were assessed with ultrasonic extraction and hot water extraction, separately, and the regression coefficient between trigonelline contents obtained under the two extraction methods was 0. 996 4. The method is simple, rapid, highly sensitive, and suitable for the analysis of trigonel-line and quality control of coffee samples.关键词
超声波萃取/高效液相色谱/葫芦巴碱/咖啡粉/速溶咖啡Key words
ultrasonic extraction/ high performance liquid chromatography ( HPLC )/ trigonel-line/ coffee powder/ instant coffee分类
化学化工引用本文复制引用
刘宏程,黎其万,邵金良,严红梅,兰珊珊..超声波萃取-高效液相色谱测定咖啡粉和速溶咖啡中的葫芦巴碱[J].色谱,2011,29(11):1103-1106,4.基金项目
云南咖啡产业技术标准体系建设基金项目(2010-009)和云南省地方标准建立基金项目. （2010-009）
