色谱2011，Vol.29Issue(11)：1141-1144,4.DOI:10.3724/SP.J.1123.2011.01141
在线柱浓缩-超高效液相色谱法测定水体中的痕量甲萘威和呋喃丹
Determination of trace carbaryl and carbofuran in water by online column enrichment-ultra high performance liquid chromatography
摘要
Abstract
An online column enrichment-ultra high performance liquid chromatography ( UH-PLC ) method was developed to determine trace carbaryl and carbofuran in water. The sample was injected into a UHPLC system directly after filtration with 0.22 μm membrane, and then enriched by online solid phase extraction ( SPE ) column. The analyte was back-flushed into the analytical column Acclaim RSLC C18 ( 100 mm ×2. 1 mm, 2. 2 μm ) by valve switching method. The mobile phases were 10 mmol/L ammonium acetate buffer ( pH 5.0, adjusted by acetic acid ) and acetonitrile in a gradient elution mode with a flow rate of 0. 8 mL/min, and detected by a diode array detector with the detection wavelength of 280 nm. The good linear ranges of carbaryl and carbofuran were 1.0- 100 μg/L with the correlation coefficients ( r2 ) larger than 0. 999 9, and the limits of detection ( S/N = 3 ) were 0. 5 μg/L and 0. 25 μg/L, respectively. The average spiked recoveries were in the range of 76. 0% - 120. 0%. The method has been applied to determine trace carbaryl and carbofuran in water samples with satisfactory results.关键词
双梯度/在线柱浓缩/超高效液相色谱/甲萘威/呋喃丹Key words
dual gradient/ online column enrichment/ ultra high performance liquid chroma-tography ( UHPLC )/ carbaryl/ carbofuran分类
化学化工引用本文复制引用
王恩智,杨新磊,叶明立,汪琼,蔡小军..在线柱浓缩-超高效液相色谱法测定水体中的痕量甲萘威和呋喃丹[J].色谱,2011,29(11):1141-1144,4.基金项目
浙江省自然科学基金项目(Nos.R4080124,Y507252)和浙江省分析测试基金项目(No.2007F70061). （Nos.R4080124,Y507252）
