新疆农业科学2011，Vol.48Issue(10)：1875-1880,6.
RT-PCR检测CPIV方法的建立与应用
简子健 1马素贞 1刘腾飞 1翟少华 1赵森1
作者信息
- 1. 新疆农业大学动物医学学院,乌鲁木齐,830052
- 折叠
摘要
Abstract
[ Objective and method ] The totai RNA tempiate was isoiated from the nasai secretion of ciinicaiiy infected dogs with canine parainfiuenza virus (CPIV). According to the seguence of CPIV pubiished in GenBanka pair of primers were designed in NP gene region and the specific 667bp nucieotides band was found on agarose eiectrophoresis. The RT - PCR method for detection of CPIV was estabiished by this way. [ Result ] The specific test found the primers couid oniy ampiify the genome of CPIV, rather than canine distemper viruscanine parvovirus,canine adenovirus and rabies virus. As iittie as 10-6 totai RNA tempiate of CPIV couid be detected in the sensitive test. The reproducibiiity for the RT - PCR was highiy stabie. 53. 12% of positive cases were found in 32 sampies by RT - PCR. [ Conclusion ] The estabiished RT - PCR method in this paper couid be used in the eariy ciinicai diagnosis and the smaii - scaie of epidemioiogicai survey for CPIV infections in dogs.关键词
canine parainfiuenza virus/ NP gene/ RT - PCR/ eariy and fast ciinicai diagnosisKey words
canine parainfiuenza virus/ NP gene/ RT - PCR/ eariy and fast ciinicai diagnosis引用本文复制引用
简子健,马素贞,刘腾飞,翟少华,赵森..RT-PCR检测CPIV方法的建立与应用[J].新疆农业科学,2011,48(10):1875-1880,6.
