中国病理生理杂志2011，Vol.27Issue(12)：2291-2295,5.DOI:10.3969/j.issn.1000-4718.2011.12.010
结缔组织生长因子在高糖诱导的足细胞上皮间充质转化中的作用
Role of connective tissue growth factor in high glucose-induced epithelial-mesenchymal transition in podocytes
摘要
Abstract
AIM: To investigate the role of connective tissue growth factor ( CTGF ) in high glucose - induced epithelial - mesenchymal transition ( EMT ) in podocytes. METHODS: The differentiated podocytes cultured under different conditions for 24 h at 37 °C were divided into 4 groups: control ( 5 mmol/L glucose solution ), 5 mmol/L glucose solution supplemented with 50 μg/L CTGF, high glucose ( 30 mmol/L glucose solution ) and high glucose supplemented with CTGF ( 50 μg/L ). The morphology of cultured podocytes was observed under phase - contrast microscope. To study the relevant markers of EMT, the Mrna and protein expression was analyzed by real - time RT - PCR and Western blotting, respectively. In addition, the effect of CTGF inhibition with anti - CTGF antibody on high glucose - induced EMT in podocytes was investigated. RESULTS: High glucose induced phenotypic transition in the podocytes from an arborization morphology to a cobblestone morphology. After addition of CTGF to high glucose culture, the podocytes developed a feature of spindle. Under high glucose condition, podocytes underwent EMT, as the epithelial marker ( nephrin ) was decreased and the mesenchymal marker ( desmin ) was increased. Exogenous CTGF showed the effect of synergy on high glucose - induced EMT. Moreover, high glucose - induced EMT in podocytes was prevented by CTGF inhibition with anti - CTGF antibody. CONCLUSION: CTGF is involved in high glucose - induced EMT in podocytes. Inhibition of CTGF might prevent podocytes from injury in diabetes.关键词
结缔组织生长因子/高糖/足细胞/上皮间充质转化Key words
Connective tissue growth factor/ High concentration of glucose/ Podocytes/ Epithelial-mesenchymal transition分类
医药卫生引用本文复制引用
戴厚永,汤日宁,郑敏,倪杰,马坤岭,刘必成..结缔组织生长因子在高糖诱导的足细胞上皮间充质转化中的作用[J].中国病理生理杂志,2011,27(12):2291-2295,5.基金项目
国家自然科学基金资助项目 (No.30870953) （No.30870953）
