中国病理生理杂志2011，Vol.27Issue(12)：2296-2301,6.DOI:10.3969/j.issn.1000-4718.2011.12.011
高糖对大鼠晚期内皮祖细胞增殖、迁移、黏附及分泌功能的影响
Effects of high glucose on proliferation, migration, adhesion and secretion of rat late endothelial progenitor cells
摘要
Abstract
AIM: To investigate the effects of high glucose on the proliferation, adhesion, migration and secretion potentials of late endothelial progenitor cells ( EPCs ) from bone marrow. METHODS: Mononuclear cells were collected from rat bone marrow by density gradient centrifugation and cultured with M199 medium. The early EPCs were identified by Dil - ac - LDL and FITC - UEA - 1 double staining. The late EPCs were identified by RT - PCR to detect the expression of von Willebrand factor( vWF ) and VE - cadherin. Moreover, the cells were identified by FACS to detect the expression of CD133 and vascular endothelial growth factor receptor - 2( VEGFR - 2 ). The 3rd generation of EPCs was harvested and incubated with glucose in a series of concentrations ( 5, 10, 20 or 40 mmol/L ). The cell proliferation, adhesion, migration and the secretion of chemokines such as monocyte chemoattractant protein - l( MCP - 1 ) and interleukin - 8 ( IL - 8 ) were assayed with MTT, adhesion test, modified Boyden chamber assay and ELISA, respectively. RESULTS: Compared with normal glucose ( 5 mmol/L )treatment, high glucose (10, 20, 40 mmol/L ) dose - dependently degraded the proliferation and migration of late EPCs ( P <0. 05 or P <0. 01 ). At the same time, treatment with glucose at the concentration of 40 mmol/L decreased the adhesion of EPCs ( P < 0. 05 ) and increased the release of MCP - 1 and IL - 8 by late EPCs. CONCLUSION: High glucose inhibits proliferation, adhesion and migration of late EPCs, and enhances the secretion of inflammatory factors, indicating that the high glucose correlates with the vascular complications of patients with diabetes.关键词
葡萄糖/晚期内皮祖细胞/糖尿病Key words
Glucose/ Late endothelial progenitor cells/ Diabetes mellitus分类
医药卫生引用本文复制引用
李岩,唐可欣,李宏,张杰,成敏..高糖对大鼠晚期内皮祖细胞增殖、迁移、黏附及分泌功能的影响[J].中国病理生理杂志,2011,27(12):2296-2301,6.基金项目
教育部新世纪优秀人才支持计划(No.NCET-10-0922) （No.NCET-10-0922）
山东省教育厅资助项目(No.J09LF06) （No.J09LF06）
