中国病理生理杂志2011，Vol.27Issue(12)：2333-2337,5.DOI:10.3969/j.issn.1000-4718.2011.12.018
大豆异黄酮对去势雌大鼠缺血再灌注脑组织细胞凋亡及calpain表达的影响
Effects of soybean isoflavones on calpain expression and neuronal apoptosis in ischemic and reperfused brain tissues of ovariectomized female rats
摘要
Abstract
AIM: To study the effects of soybean isoflavones on neuronal apoptosis and mRNA expression of calpain in transient focal cerebral ischemia and reperfusion ( FCIR ) rats. METHODS: Thirty - six adult healthy female SD rats were ovariectomized and randomly divided into soybean isoflavone - treated group and control group. The rats in soybean isoflavone - treated group were subdivided into low and high doses of soybean isoflavone groups. After 1 month of treatment, a FCIR model was established by intraluminal middle cerebral artery occlusion ( MCAO ) with a nylon monofila-ment suture. In situ hybridization was performed to examine the mRNA expression of calpain. Apoptosis was observed with terminal deoxynucleotidyl transferase - mediated dUTP nick - end labeling ( TUNEL ). The activity of calpain was also examined. RESULTS: The mRNA expression of calpain and TUNEL positive cells were observed in the cortex and striatum of ischemic hemisphere. Calpain mRNA was present at lower levels in soybean isoflavone - treated groups than that in control group ( P <0. 05 ). The number of apoptotic cells decreased in soybean isoflavone - treated groups compared with control group ( P < 0. 05 ). No significant difference of the calpain mRNA and the number of apoptotic cells between high -dose and low - dose soybean isoflavone - treated groups was observed ( P <0. 05 ). The activity of calpain was also reduced in soybean isoflavone -treated groups. CONCLUSION: Soybean isoflavones attenuate ischemic neuronal apoptosis by reducing the activity and inhibiting the mRNA expression of calpain.关键词
大豆异黄酮/Calpain/细胞凋亡/大鼠Key words
Soybean isoflavones/ Calpain/ Apoptosis/ Rats分类
医药卫生引用本文复制引用
么晓轶,于纪珠,李颖..大豆异黄酮对去势雌大鼠缺血再灌注脑组织细胞凋亡及calpain表达的影响[J].中国病理生理杂志,2011,27(12):2333-2337,5.基金项目
黑龙江省卫生厅基金资助项目(No.2006-161) （No.2006-161）
