中国病理生理杂志2011，Vol.27Issue(12)：2357-2361,5.DOI:10.3969/j.issn.1000-4718.2011.12.022
TGF-β1在雷奈酸锶促进大鼠骨髓间充质干细胞向成骨细胞分化中的作用
Role of TGF-β1 in strontium ranelate-induced osteogenic differentiation of rat bone marrow mesenchymal stem cells
摘要
Abstract
ADM: To study the role of transforming growth factor β1( TGF - β1 ) in strontium ranelate ( Sr ) - induced osteogenic differentiation of rat bone marrow mesenchymal stem cells( rBMSCs ). METHODS: The activity of alkaline phosphatase( ALP ) and the number of calcium nodules were detected by commercial kits. The expression of TGF -β1 was detected by Western blotting. RESULTS: Exposure of rBMSCs to Sr at the concentrations from 0. 1 mmol/L to 7 mmol/L for 7 days obviously increased activity of ALP. When the concentration of Sr was up to 3 mmol/L, the activity of ALP reached to the highest level( P <0. 01 ). Treatment of rBMSCs with Sr at the same concentration for 21 days also significantly increased the number of calcium nodules. When rBMSCs were treated with Sr at 0. 1 mmol/L to 5 mmol/L for 7 days,the expression of TGF - β1 z was enhanced obviously. When the concentration of Sr was up to 1 mmol/L, the expression of TGF - β1 reached to maximum. For 1 to 7 days, 1 mmol/L Sr time - dependently increased the expression of TGF - β1 . Pretreatment of rBMSCs with SB431542, an inhibitor of TGF - β1 , for 2 h, prior to exposure to Sr( 1 mmol/L ), inhibited not only Sr - induced overexpression of TGF - p, ,but also the activity of ALP and the number of calcium nodules. CONCLUSION : Sr promotes rBMSCs differentiating into osteoblasts by up - regulating the expression of TGF - β1关键词
雷奈酸锶/骨髓间充质干细胞/转化生长因子β/成骨分化Key words
Strontium ranelate/Bone marrow mesenchymal stem cells/Transforming growth factor beta/ Osteo-blastic differentiation分类
医药卫生引用本文复制引用
王小娜,李正,王瑒,兰爱平,吴文..TGF-β1在雷奈酸锶促进大鼠骨髓间充质干细胞向成骨细胞分化中的作用[J].中国病理生理杂志,2011,27(12):2357-2361,5.基金项目
十一五国家科技支撑项目(No.2006BAI02B03) （No.2006BAI02B03）
广东省科技计划项目(No.2008B060600039) （No.2008B060600039）
广东省科技计划项目(No.2009B030801261) （No.2009B030801261）
