中国病理生理杂志2011，Vol.27Issue(12)：2372-2375,4.DOI:10.3969/j.issn.1000-4718.2011.12.025
冬虫夏草对慢性阻塞性肺疾病大鼠树突状细胞功能的影响
Influence of Cordyceps sinensis on cytokine profile of dendritic cells in chronic obstructive pulmonary rat model
摘要
Abstract
AIM: To investigate the effect of Cordyceps sinensis ( CS ) on dendritic cells ( DCs ) in the rat model of chronic obstructive pulmonary disease ( COPD ). METHODS: Eighteen Sprague - Dawley male rats were randomly divided into 3 groups: control group, COPD group and CS group. The rats in the latter 2 groups were exposed to cigarette smoking for 8 weeks with ( CS group ) or without ( COPD group ) CS treatment. The rats in control group were maintained under normal condition. After 8 weeks, the histological changes of the right lung were observed under microscope. The DCs from the 3 groups were harvested and the supernatants of DCs were analyzed for the levels of TNF - a and IL - 12 p70 by commercially available ELISA kit. The DCs were then washed and cocultured in vitro with autologous T cells purified by a nylon cotton column. The supernatants of DCs - T coculture were collected after 72 h incubation, and analyzed for the levels of interleukin - 5 ( IL - 5 ) and interferon - 7 ( IFN - 7 ) by ELISA. RESULTS: Analysis of the rat lung parenchyma revealed a significant decrease in the mean alveolar number, an indicator of alveolar density, in COPD group ( 38 ± 16 ) and CS group ( 48 ± 9 ) in comparison with control group ( 62 ± 8 ). The mean alveolar number tended to be increased in CS group than that in COPD group, although this difference did not achieve statistical significance ( P > 0. 05 ). The concentrations of TNF - a and IL - 12 p70 in the culture supernatants of DCs and IFN - 7 in the supernatants of DCs - T co-cluture were up - regulated in CS group as compared with those in COPD group and control group ( P < 0.05 ). The level of IL - 5 in the DCs - T coculture supernatants of the 3 groups did not show differences with statistical significance ( P > 0. 05 ). CONCLUSION: The therapeutic effects of CS on COPD rats may be related to modulation of Thl and Th2 cell functions. This effect is probably mediated through IL - 12 p70 produced by DCs and Thl cytokine IFN - 7 produced by autologous T cells.关键词
肺疾病,慢性阻塞性/冬虫夏草/树突状细胞Key words
Pulmonary disease, chronic obstructive/ Cordyceps sinensis/ Dendritic cells分类
医药卫生引用本文复制引用
李洪涛,张天托,黄静,刘慧,川上和羲..冬虫夏草对慢性阻塞性肺疾病大鼠树突状细胞功能的影响[J].中国病理生理杂志,2011,27(12):2372-2375,4.基金项目
广东省中医药局基金资助项目(No.2009156) （No.2009156）
广东省医学科学基金资助项目(No.A2009194) （No.A2009194）
