摘要
Abstract
0bJ3ctive:To evaluate 1he influence of Bacille Cam ette Guerin (BCG )team ent on 1he ahway inflammation ,1he cytokines in bronchoalveolar lavage fluid and expressbn of signal transducer and activator of transcription 6 (STAT6)in lung of asihm atic m ouse .M e1h- ods:30 Kunm ing m ice were randan fy divided inti contol group ,as1hm a group and BCG teatn ent group .The m ice were sensitized and challenged w ith ovalbum in (OVA ) ti establish asthm atic m odel.The m ice in BCG teatn ent group w ere inJ3cted intradem alfy w ilh BCG 5 days be- ibre and after antigen sensitizatfan.After1he finalOVA inhalation ,ti assanble iheBALF and of mice at 24 h.To count 1he number of trial cells and eosinophils in BALF .HE stained to observe lung inflammation .The concentration of cytokine interleukin 4(L~4) and inteiferon y(FN-y) in BALF were detected by engine linked in munosorbent assay (ELBA).The expressions of STAT6 in lungs were detected by inmunohistoche- mistry.ResulteiHE stained showed 1hat1he asthm a group were infiltrated by generous inflammatory cell.The number of trial cells,EOS ,L-4 in BALF and STAT6 in lung in asthm a group were higher 1han 1hose in control group (P<0 .05).The number of total cells, EOS ,JtA in BALF and STAT6 in lung in BCG team ent group were higher 1han 1hose in control group ( P<0 .05) .The number of total cells,EOS ,L~4 in BALF and STAT6 in lung BCG team ent group w ere low er 1han 1hose in asthm a group ( P<0 .05 ) .Can pared w ith control group ,1he level of FN-^y was significant^ decreased in asthm a group (P<0 .0l),however,1he level of FN-^y was significant^ higher in BCG teatn ent group (P<0 . 01) . Conclusion :BCG can inhibit 1he airway inflammation of asthm atic mouse, decrease 1he production ofTh2 cytokines like L~4 and elevate Th2 cytokines such as FN-^y ,1hose m ay ow ing to BCG inhibit 1he expression of STAT6 in lung of asftm atic m ouse.关键词
哮喘/卡介苗/信号转导和转录激活因子-6/IL-4/IFN-γKey words
AsthmajBacille Cain ette Guerin /Signal transducer and activator of transcription 6 jlhterleukin 4/lhterferon y分类
医药卫生
