色谱2011，Vol.29Issue(11)：1128-1132,5.DOI:10.3724/SP.J.1123.2011.01128
高效毛细管电泳法检测牛奶中的青霉素中间体以及3种青霉素类药物
Determination of penicillin intermediate and three penicillins in milk by high performance capillary electrophoresis
摘要
Abstract
A high performance capillary electrophoresis ( HPCE ) method was developed for the simultaneous determination of penicillin intermediate and penicillins in milk, including 6-amino-penicillanic acid ( 6-APA), penicillin G ( PEN ), ampicillin ( AMP ) and amoxicillin ( AMO ). The main parameters including the ion concentration and pH value of running buffer, separation voltage and column temperature were optimized systematically by orthogonal test. The four penicillins ( PENs ) were baseline separated within 4. 5 min with the running buffer of 40 mmol/L potassium dihydrogen phosphate-20 mmol/L borax solution ( pH 7. 8 ), separation volt-age of 28 kV and column temperature of 30 ℃. The calibration curves showed good linearity in the range of 1. 56 - 100 mg/L, and the correlation coefficients ( r2 ) were between 0. 997 9 and 0.999 8. The average recoveries at three spiked levels were in the range of 84.91% -96.72% with acceptable relative standard deviations ( RSDs ) of 1. 11% -9. 11%. The method is simple, fast, accurate and suitable for the determination of penicillins in real samples.关键词
高效毛细管电泳/6-氨基青霉烷酸/青霉素中间体/青霉素类药物/牛奶Key words
high performance capillary electrophoresis( HPCE )/ 6-aminopenicillanic acid/ penicillin intermediate/ penicillins/ milk分类
化学化工引用本文复制引用
田春秋,檀华蓉,高丽萍,沈虎琴,祁克宗..高效毛细管电泳法检测牛奶中的青霉素中间体以及3种青霉素类药物[J].色谱,2011,29(11):1128-1132,5.基金项目
"十一五"国家科技支撑项目(2006BAK02A08_4). （2006BAK02A08_4）
