中国免疫学杂志2011，Vol.27Issue(12)：1059-1061,1065,4.DOI:10.3969/j.issn.1000-484X.2011.12.001
乙肝疫苗无应答者CD4+T细胞TCR Vβ基因克隆化特征的研究
Analysis of CD4+T cell TCR gene in non responders for hepatitis B vaccine
摘要
Abstract
0bJ3ctive:To study 1he characteristics of CD4 T cells TCR VfS gene cbnning in hepatitisB vaccinated and 1he difference of TCR Vβ genes m onocbnal changes beteeen 1he cbne of genes in respondeis and non responders 1o hepatitis B vaccine .M e1hods:CD4+ T cells of 80 hepatitisB vaccinated individualV(3 genes CDR3 regbn fragmentswere amplified by multi-prmerPCR empUScation m eihods,PCR products w ere aplied on agarose gel etectophoresis and gene scanning by sequencing instnm ent.R esulte :From 80 cases of hepatitis B vaccinat-ed indiridual,58 cases were positive fbrantrHBs and 22 cases were negative .M onocbnal TCR VfS gene changes w ere m ain^r concentrated in V(& ,V(I ,V(I ,V(li ,and V(l7 fim % of genes .The frequency of changes in 1hese fire fen % of genes was significant^ bwer in nonrespondeis group 1han responders (P<0 .01 or P<0 .05) . Condusbn :CD4+ T cells of TCR V(3 gene change is one of 1he in portant iactir 1hat affect 1he body's inmune response to hepatitis B vaccine.关键词
乙肝疫苗/T细胞抗原受体/免疫应答Key words
H epatitis B vaccine /T cell antigen recept>r(TCR ) / In m une response分类
医药卫生引用本文复制引用
宋玉国,熊英,宋宇,毕胜利..乙肝疫苗无应答者CD4+T细胞TCR Vβ基因克隆化特征的研究[J].中国免疫学杂志,2011,27(12):1059-1061,1065,4.基金项目
本文为国家自然科学基金(No.30772026)和吉林省科技发展计划(20090942)资助项目 （No.30772026）
