山西医科大学学报2011,Vol.42Issue(12):967-969,3.DOI:10.3969/J.ISSN.1007-6611.2011.12.008
HPLC测定肤痒胶囊中羟基红花黄色素A的含量
Determination of the content of hydroxysafflor A in Fuyang capsule by high performance liquid chromatography
王增仙 1李艳丽 1高萧枫 1武敏霞 1甄会贤 1李平1
作者信息
- 1. 山西生物应用职业技术学院生物制药工程系,太原,030031
- 折叠
摘要
Abstract
Objective To establish a high performance liquid chromatography( HPLC ) method for determining the content of hydrox-ysafflor A( HYSA ) in Fuyang capsule. Methods Hypersil ODS was applied with the mobile phase of methanol-acetonitrile-0. 7% phosphoric acid ( 20: 2: 78 ) and UV detection wavelength of 403 nm. The content of HYSA in Fuyang capsule was determined by HPLC method. Results HYSA showed a good linear relationship in the range of 1. 38 - 13. 8 μg. The average recovery was 101. 83% and RSD was 0. 2% ( n =5 ). Conclusion The established method is accurate and reliable,and could be used for the quality control of the Fuyang capsule.关键词
肤痒胶囊/羟基红花黄色素/HPLCKey words
Fuyang capsule/hydroxysafflor A/high performance liquid chromatography分类
医药卫生引用本文复制引用
王增仙,李艳丽,高萧枫,武敏霞,甄会贤,李平..HPLC测定肤痒胶囊中羟基红花黄色素A的含量[J].山西医科大学学报,2011,42(12):967-969,3.