国际眼科杂志2012，Vol.12Issue(1)：4-6,3.DOI:10.3969/j.issn.1672-5123.2012.01.02
大鼠视网膜神经细胞的原代培养
Primary cultivation of retinal neurons of neonatal rat
阴杰平 1孟岩 1陈召利1
作者信息
- 1. 266003,中国山东省青岛市,青岛大学医学院附属医院眼科
- 折叠
摘要
Abstract
·AIM: To explore an experimental method for primary culture of retinal neurons of neonatal rat.·METHODS: Retina of postnatal 1-3 days SD rats was dissected into cell suspension by using trypsin digestion and cultured in vitro with DMEM/F12. Immunocytochemical methods were used to identify the cultured neurons.·RESULTS: All cultured cells underwent adherence and some possessed axons，of which some were connected with each other．Most cells were neuron specific enolase(NSE)-positive detected by immunohistochemistry．·CONCLUSION: Successful culture of retinal neuron cells in vitro is helpful in the research of retinal diseases.关键词
细胞培养/视网膜神经元/胰蛋白酶消化法/神经元特异性烯醇化酶Key words
cell culture/retinal neuron/trypsin digestion/neuron specific enolase引用本文复制引用
阴杰平,孟岩,陈召利..大鼠视网膜神经细胞的原代培养[J].国际眼科杂志,2012,12(1):4-6,3.
