军事医学2011，Vol.35Issue(11)：862-866,5.
肠道病毒71型(EV71)ICR乳鼠动物模型的建立
Animal models on ICR mice with EV71 infection
刘娟 1李康 2杨静 1王升启2
作者信息
- 1. 北京工业大学生命科学与生物工程学院,北京100124
- 2. 军事医学科学院放射与辐射医学研究所,北京,100850
- 折叠
摘要
Abstract
Objective To create possible enterovirous 7l( EV71 )ICR mice animal models for the identification of the pathogenetic mechanism and vaccine evaluation. Method ICR mice of different age were infected with EV71 virus through the intraperitoneal( IP )route. The organ and tissue of the dying mice were collected at various time points for pathogeny diagnoses. Meanwhile, one step RT-PCR method was developed in our study. Results The ICR mice infected through the intraperitoneal( IP )route showed severe clinical symptoms,such as cachexia, low movement, paralysis, low body mass and death. Virus RNAs could be detected by RT-PCR in the lung, brain and intestine in the neonate mice that were infected through the IP route. Virus RNAs were detected by RT-PCR in the lung, brain, intestine and leg muscle in the seven-day mice. The moleculer biology method developed could be used for the further study of other animals in the future. Conclusion The infection of ICR mice( one-day mice, one-week mice, or three-week mice ) through the IP route proves to be at different levels using EV71 strain isolated from patients. The outcome of infection of ICR mice can be used for pathogenetic research as an animal model despite some instability.关键词
肠道病毒A型,人/EV71病毒/ICR小鼠/手足口病/动物模型Key words
enterovirus A/human/EV71 virus/ICR mice/hand-foot-mouth disease/animal models分类
医药卫生引用本文复制引用
刘娟,李康,杨静,王升启..肠道病毒71型(EV71)ICR乳鼠动物模型的建立[J].军事医学,2011,35(11):862-866,5.
