发光学报2011，Vol.32Issue(11)：1197-1203,7.
喜树碱与DNA相互作用的光谱学研究
Spectroscopic Study of The Interaction between Camptothecin and DNA
于岚岚 1杨冉 1白希希 1陈晓英 1李建军 1屈凌波1
作者信息
摘要
Abstract
The interaction of an anti-cancer drug camptothecin with salmon sperm DNA was studied by spec-troscopic techniques. DNA induced slight hypochromic and bathochromic effects on the UV-Vis absorption spectra of camptothecin, but strong fluorescence quenching of camptothecin, which suggest the formation of ground-state camptothecin-DNA complex. The binding constant and number of binding site were calculated and the main interaction force was determined. The effect of salt, phosphate and negatively charged quencher KI on camptothecin-DNA interaction was investigated and the interaction of camptothecin with double-stranded DNA and single-stranded DNA was compared. The melting temperature of camptothecin-DNA complex was 60 ℃ , which is 18℃ lower than that of pure DNA. All the results indicate that groove binding mode is the main interaction mode between camptothecin and DNA.关键词
camplolhecin/drug-DNA inleraclion/fluorescence quenchingKey words
camplolhecin/drug-DNA inleraclion/fluorescence quenching引用本文复制引用
于岚岚,杨冉,白希希,陈晓英,李建军,屈凌波..喜树碱与DNA相互作用的光谱学研究[J].发光学报,2011,32(11):1197-1203,7.
