中国药业2011，Vol.20Issue(24)：46-47,2.
反相高效液相色谱法测定硫酸阿托品注射液的含量
Determination of Aatropine Sulfate Injection by RP-HPLC Method
刘海燕 1蒋红霞1
作者信息
- 1. 河南省周口市食品药品检验所,河南,周口,466000
- 折叠
摘要
Abstract
Objective To establish a HPLC method for determining the content of Atropine Sulfate Injection. Methods The RP - HPLC method was adopted. The column was Kromasil C18 column (250 mm × 4.6 mm, 5 μm), the mobile phase was 0.05 mol/L potassium dihydrogen phosphate solution (containing 0.002 5 mol/L heptane sodium sulfonate) - acetonitrile(82 : 18) (adjusting pH 5.0 with sodium hydroxide solution), the flow rate was 0. 8 mL/min and the detection wavelength was 225 nm. Results The linear range was 1. 002 4 -10. 024 μg( r = 0.999 75). The average recovery rate was 99. 81%, RSD =0. 79% (n = 6). Conclusion The method is simple,rapid,accurate and suitable for the quality control of Atropine Sulfate Injection.关键词
反相高效液相色谱法/硫酸阿托品注射液/含量/酸性染料比色法Key words
RP - HPLC/ Atropine Sulfate Injection/ content/ acid dye colorimetry分类
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刘海燕,蒋红霞..反相高效液相色谱法测定硫酸阿托品注射液的含量[J].中国药业,2011,20(24):46-47,2.
