中国中西医结合杂志2011，Vol.31Issue(8)：1097-1100,4.
解毒化瘀方含药血清对K562/A02细胞凋亡抑制蛋白及核周因子κB表达的影响
Effect of Jiedu Huayu Recipe Containing Serum on the Expressions of Survivin and NF-κB of K562/A02 Cell Apoptosis by
摘要
Abstract
Objective To study the effect of Jiedu Huayu Recipe containing serum on the expressions of survivin gene of leukemia K562/A02 cell apoptosis and the expression of nuclear factor kappa B ( NF-kB) . Methods K562/A02 cells were divided into six groups. An equal volume of calf serum, rabbit serum, high, middle, and low dose Jiedu Huayu Recipe containing serum, and interferon was respectively added. K562 sensitive cell strain was set up as the control. The expressions of survivin in K562/A02 cells after treated with Jiedu Huayu Recipe containing serum were detected using semi-quantitative reverse transcriptase polymerase chain reaction method. The expressions of NF-κB/P65, NF-kB/P50, and IκBα were detected using Western blot method. Results The survivin resistant gene was highly expressed in K562/A02 cells. After treated by Jiedu Huayu Recipe containing serum, the expressions of survivin could be obviously lowered in the high and middle dose Jiedu Huayu Recipe containing serum groups as well as the interferon control group. NF-kB was also highly expressed in K562/A02 resistant cells. After treated by Jiedu Huayu Recipe containing serum, the expressions of P65, P50, and IκBα decreased to some degrees. The decrement was the most obvious in the middle and high dose Jiedu Huayu Recipe containing serum groups. The decreased expressions of P65 and P50 in the high dose Jiedu Huayu Recipe containing serum group were higher than that in the interferon control group (P<0. 05). The decrement of IκBα was equivalent to that of the interferon control group (P>0.05). Conclusion Jiedu Huayu Recipe could block the NF-kB signal pathway of K562/A02 cells and reverse drug resistance by influencing the expression of NF-KB-survivin genes.关键词
白血病/多药耐药/K562/A02细胞/survivin/核周因子κB信号/解毒化瘀方Key words
leukemia/ multi-drug resistance/ K562/A02 cell/ survivin/ NF-kB signal/ Jiedu Huayu Recipe引用本文复制引用
马武开,李玉莹,姚血明,黄礼明,姚宇红..解毒化瘀方含药血清对K562/A02细胞凋亡抑制蛋白及核周因子κB表达的影响[J].中国中西医结合杂志,2011,31(8):1097-1100,4.基金项目
国家自然科学基金资助项目(No.30860353) （No.30860353）
