水产学报2011，Vol.35Issue(8)：1121-1129,9.DOI:10.3724/SP.J.1231.2011.17489
鳜T细胞表面受体CD4分子的克隆与表达分析
The cDNA sequence and expression analysis of CD4 in mandarin fish (Siniperca chuatsi)
摘要
Abstract
The Cdna sequence of CD4 homologue was cloned from the mandarin fish Siniperca chuatsi. It consists of 2 356 bp,encoding 469 amino acids,and this molecule has a typical four Ig-like domains( Vl-Cl-V2-C2)and shares some fundamental conserved structures,I. E. WTC motif in D2 domain, Af-glycosylation site in D4 domian and the CXC motif in the cytoplasmic tail. Unlike avian and mammalian CD4 molecules, the mandarin fish CD4 sequence lacks the Cys pairs in the first Ig-like domain, but has a unique possible disulfide bond in the third domain. These differences suggest that mandarin fish CD4 molecule may have a different structure, which may in turn influence its binding with MHC II molecule and probably subsequent T-cell activation. The CD4 was expressed mainly in thymus, and following stimulation with LPS, its significant increase(P <0. 05) at Mrna level was observed 8 hours post injection in thymus, spleen and head-kidney. The mandarin fish CD4 shared a relatively high level of similarity(57% ) with that in sea bass Dicentrarchus labrax,and in the phylogenetic tree,the mandarin CD4 was clustered with that in sea bass. The cloning of CD4 in the mandarin fish may serve as a basis for further functional characterization of fish T lymphocytes.关键词
鳜/T细胞/CD4/表达Key words
mandarin fish (Siniperca chuatsi) / T lymphocyte/ CD4/ expression分类
生物科学引用本文复制引用
郭政,聂品..鳜T细胞表面受体CD4分子的克隆与表达分析[J].水产学报,2011,35(8):1121-1129,9.基金项目
国家自然科学基金委与广东省联系基金(U0631010) （U0631010）
