云南农业大学学报2011，Vol.26Issue(5)：678-682,5.DOI:10.3969/j.issn.1004-390X(n).2011.05.018
秋水仙碱诱导铁皮石斛多倍体研究初报
Study on the Use of Colchicine to Induce Polyploidy of Dendrobium candidum Wall.ex Lindl
摘要
Abstract
To induce polyploidy, the protocorm-like bodies and caespitose shoots of Dendrobium candi-dum were soaked with different concentrations of colchicine solution and different treating times. The results showed that the optimum condition was soaking shoots in 0. 09% colchicine solution for 24h, the highest induction rate was about 48% and the death rate was 10%. The characteristics of leaves, stoma and the number of chromosome of treated plants were checked and some plants were determined to be tetraploid, others were different chimeras needed to be separated, tetraploid were distinctly different from diploid. The number of chromosomes of tetraploids cells was 2n =4x =76, while that of dip-loid cells was 2n = 2x = 38.关键词
铁皮石斛/多倍体/秋水仙碱/染色体Key words
Dendrobium candidum/ polyploid/colchicine/ chromosomes分类
园艺学与植物营养学引用本文复制引用
张青华,李枝林,唐敏,许春城,席会鹏..秋水仙碱诱导铁皮石斛多倍体研究初报[J].云南农业大学学报,2011,26(5):678-682,5.基金项目
云南省科技基金项目(2009BB013). （2009BB013）
