安徽农业科学2011，Vol.39Issue(27)：17010-17012,3.
AccQ-Tag-HPLC法测定烟叶生长过程中游离氨基酸的变化
Detection of Free Amino Acids in Tobacco Leaves during Growth Process by Using AccQ-Tag-HPLC Method
摘要
Abstract
[Objective] The study aimed to establish a method for the detection of free amino acids in tobacco by using AccQ-Tag-HPLC sys tem. [ Method] With the AccQ-Tag reagent bag from WATERS company as the basic condition of building method, through the optimization of pretreatment and chromatographic conditions for the free amino acid in the tobacco, the 6-amino decoquinate-N-hydroxyl succinyl imide formic ether (AQC) was taken as the derivative reagent to make for the column quantitative derivatization with each amino acid and then to elute by using AccQ-TagTM column gradient, thus, a AccQ-Tag-HPLC determining method for the detection of free amino acids in tobacco was estab lished. [ Result ] When the method was used to determine the free amino acids in the kill out samples of tobacco, 16 kinds of free amino acids in tobacco leaves were separated and detected by the method, with the recovery of 82. 8% - 105. 3% and the standard deviation values of be low 2.5% , showing a better reproducibility and recovery. The contents of proline, aspartate, serine and alanine in free amino acids in the kill out samples of the flue-cured tobacco were higher. [ Conclusion ] AccQ-Tag-HPLC method was a kind of practical method for the analysis of the free amino acid in the tobacco, which was suitable for the determination on the content variation of free amino acid in the tobacco in the different periods from the vigorous growing stage to maturity.关键词
烟草/游离氨基酸/AccQ-Tag高效液相色谱法Key words
Tobacco/ Free amino acid/ AccQ-Tag-HPLC分类
化学化工引用本文复制引用
李方楼,姬小明,魏跃伟,杨永锋,刘国顺..AccQ-Tag-HPLC法测定烟叶生长过程中游离氨基酸的变化[J].安徽农业科学,2011,39(27):17010-17012,3.基金项目
国家烟草专卖局科技攻关项目(110200401003). （110200401003）
