中国农业科学2011，Vol.44Issue(19)：4058-4065,8.DOI:10.3864/i.issn.0578-1752.2011.19.016
苦瓜皂苷和多糖的连续提取工艺及其对α-葡萄糖苷酶的抑制作用
Continuous Extraction of Saponin and Polysaccharide from Momordica charantia L.and Their InhibitoryEffect on α-glucosidase
摘要
Abstract
[Objective] The technical process and suitable conditions for continuous extraction of saponin and polysaccharide from Momordica charantia L. By ultrasonic-microwave synergistic extraction were established and to evaluate their inhibition effects on a-glucosidase [ Method ] Quadratic Regression Rotational Combinational Design was applied to optimize the extraction process of saponin and polysaccharide from Momordica charantia L. Using continuous ultrasonic-microwave synergistic extraction method. The method with substrate 4-nitrophenol-2-β-D-glucopyranoside(PNPG) was used to measure the a-glucosidase inhibitory effect of saponin and polysaccharide extract. [Result] The optimum conditions for saponin and polysaccharide extraction form Momordica charantia L. Were as follows: the dried Momordica charantia L. With 13% of water content was grinded to pass a 200 urn sieve; the solvent was 75% enthanol and the ratio of material to solvent was 1:18; ultrasonic-microwave synergistic extraction for 16 min (the power of microwave was 50 W and the ultrasonic frequency was 20 kHz). Under this condition the extraction yield of saponin from Momordica charantia L. Was 2.51%. The residue was further dissolved in water of pH 8.0 for extraction of polysaccharide, the ratioof material to solvent was 1:14; the ultrasonic-microwave synergetic extraction time was 23 min (the power of microwave was 50 W and the ultrasonic frequency was 20 kHz) at a temperature of 84 ℃. Under these conditions, the extraction yield of polysaccharide from Momordica charantia L. Was 12.86%. Both of the extracts of saponin and polysaccharide from Momordica charantia L. Showed an obvious inhibitory activity against o-glucosidase with values of 1.03 and 10.73 mg-mL-1, respectively. [ Conclusion ] The raw materials were utilized effectively and the extraction yield and efficiency were increased using a method of ultrasonic-microwave synergistic extraction. The extracted saponin and polysacchride from Momordica charantia L. Showed an obvious inhibition effect on a-glucosidase with promising of blood sugar reducing potentials.关键词
苦瓜/皂苷/多糖/连续提取/α-葡萄搪苷酶Key words
Momordica charantia L/saponin/polysaccharide/continuous extraction/α-glucosidase引用本文复制引用
王琪,邓媛元,张名位,张瑞芬,张雁,唐小俊,魏振承,池建伟..苦瓜皂苷和多糖的连续提取工艺及其对α-葡萄糖苷酶的抑制作用[J].中国农业科学,2011,44(19):4058-4065,8.基金项目
广东省自然科学基金团队项目(06200586) （06200586）
广东省农业攻关项目(2008B021100008) （2008B021100008）
