色谱2011，Vol.29Issue(9)：937-941,5.DOI:10.3724/SP.J.1123.2011.00937
云南露水草中的一种新植物甾酮的分离与分析
Isolation and analysis of a new phytoecdysteroid from Cyanotis arachnoidea C.B.Clarke
摘要
Abstract
Cyanotis arachnoidea is a plant with plenty of phytoecdysteroid. To study the active compound in it, a new phytoecdysteroid with 5a-cholesta skeleton, was isolated from the whole plant of Cyanotis arachnoidea C. B. Clarke by using various chromatographic methods (alumina column chromatography, silica gel column chromatography, octadecyl silane ( ODS) column chromatography, thin layer chromatography (TLC) and high performance liquid chromatography (HPLC)). Its structure was analyzed on the basis of ID and 2D nuclear magnetic resonances (NMR), electrospray ionization mass spectrometry ( ESI-MS) methods. It is a compound with structure of 3/3,4a,14a,20.R,22.R,25-hexahydroxy-5a-cholest-7-en-6-one, which is a rare phytoecdysteroid with 5a-H.关键词
色谱分离/核磁共振光谱/植物甾酮/露水草Key words
chromatographic isolation/ nuclear magnetic resonance ( NMR)/ phytoecdysteroid/ Cyanotis arachnoidea C. B. Clarke分类
化学化工引用本文复制引用
谭成玉,孔亮,李铣,李伟,李宁..云南露水草中的一种新植物甾酮的分离与分析[J].色谱,2011,29(9):937-941,5.基金项目
国家自然科学基金项目(21075012, 31071612)和辽宁省高等学校优秀人才支持计划项目(2009R13). （21075012, 31071612）
