色谱2011，Vol.29Issue(9)：896-900,5.DOI:10.3724/SP.J.1123.2011.00896
新型三维可调共聚焦激光诱导荧光检测器的研制与评价
Development and evaluation of a novel three-dimensional adjustable confocal laser-induced fluorescence detector
摘要
Abstract
Laser-induced fluorescence detector (LIFD) is one of the most sensitive detectors in analytical chemical files. Confocal optical configuration is widely used in LIFDs. Two effective ? Approaches used to achieve the best signal to noise ratio (S/N) are increasing the confocal precision and minimizing the background noise. A novel three-dimensional adjustable confocal LIFD was developed, using a new three-dimensional adjustable supporter of reflector and modularized optical system. A detection limit (S/N = 3) of 1 x 10~12 mol/L and a linear dynamic range of 3 orders of magnitude were obtained using fluorescein isothiocyanate (FITC) standard as the test sample. The noise level and drift levels were 8. 0 x 10 ~3 mV and 1. 4 x 10 "3 mV/h, respectively, which were almost 10 times lower than before. And the stability of the LIFD was evaluated by five replicate injections of 5 x 10"9 mol/L FITC, and the relative standard deviations (RSDs) of peak height and peak area were 0.38% and 0.41%, respectively. Further more, three biogenic amines, which were derivatized by FITC, were separated by high performance liquid chromatography (HPLC) and then detected by the novel LIFD. And the detection limits (S/N= 3) ranged 0. 01 to 0. 02 nmol/L, which were better than other methods. Therefore, the LIFD is highly sensitive, as well as shows a real low noise level and good reproducibility.关键词
激光诱导荧光检测器/三维调节架/共聚焦/评价Key words
laser-induced fluorescence detector ( LIFD ) / three-dimensional adjustable supporter/ confocal/ evaluation分类
化学化工引用本文复制引用
刘翻,于淑新,唐涛,孙元社,张维冰,李彤..新型三维可调共聚焦激光诱导荧光检测器的研制与评价[J].色谱,2011,29(9):896-900,5.基金项目
国家重点基础研究发展规划(“973”)项目(2011CB910400)子课题(2011CB910404) （“973”）
国家自然科学基金项目(21075039/B050106) （21075039/B050106）
食品科学与技术国家重点实验室2010年度开放基金项目(SKLF-KF-201002)和科技型中小企业技术创新基金项目(07C26212121171). （SKLF-KF-201002）
