深圳大学学报（理工版）2011，Vol.28Issue(5)：423-429,7.
人脑中脱碘酶相互作用.蛋白的筛选与验证
Investigation on the interactive protein of iodothyronine deiodinase 3 mutant in human brain
摘要
Abstract
Selenoproteins play important roles in maintaining cell function, resisting peroxidation damage, and preventing brain diseases. Iodothyronine deiodinase 3 (DIO3) is an important selenoprotein in the human brain. In this study, a human DIO3 gene was cloned and mutated at the TGA codon in the open reading frame encoding for a selenocysteine (Sec) residue, where the Sec was site-mutated to a cysteine residue. The DIO3' mutant was then used as a "bait" to screen a fetal brain cDNA library by yeast two-hybrid system. Preliminary investigation on the protein-protein interaction was performed by the life-time-resolved imaging measurement using the fluorescence resonance energy transfer technique. Results showed that the interactive protein of DIO3' is a serpin peptidase inhibitor (clade A, member 3).关键词
基因工程/硒蛋白/脱碘酶3/大脑/基因编码/酵母双杂交系统/蛋白质筛选/荧光共振能量转移技术Key words
genetic engineering/selenoprotein/iodothyronine deiodinases 3 (DIO3)/brain/gene encoding/yeast two-hybrid system/screening of protein/fluorescence resonance energy transfer (FRET)分类
生物科学引用本文复制引用
张毅哲,刘琼,田静,邵永红..人脑中脱碘酶相互作用.蛋白的筛选与验证[J].深圳大学学报（理工版）,2011,28(5):423-429,7.基金项目
国家自然科学基金资助项目(31070731 （）
30901182) （）
