中国畜牧兽医2011，Vol.38Issue(9)：65-68,4.
犬瘟热病毒水貂分离株F1基因的克隆表达
Cloning and Expression of F1 Gene of Mink CDV Isolate Strain
摘要
Abstract
To prepare CDV antigen for diagnosis, gene engeneering method was explored. CDV genome was extracted, Fl gene was amplified by RT-PCR cloned and then sequenced. The Fl gene was cloned into expression plasmid pET28a and expressed by the induction of IPTG inducer. The recombined protein was examined by SDS-PAGE and Western blotting,purified by Ni column. The results indicated the recombined protein could react with CDV positive serum and might be used as CDV diagnostic antigen.关键词
犬瘟热病毒/F1基因/表达Key words
canine distemper virus /F1 gene expression分类
生物科学引用本文复制引用
张蕾,柴秀丽,张大鹏,张海玲,赵建军,高晗,白雪,徐磊,闫喜军..犬瘟热病毒水貂分离株F1基因的克隆表达[J].中国畜牧兽医,2011,38(9):65-68,4.基金项目
科技部科研院所社会公益研究专项"野生动物重要传染病生态学发生,监测和控制技术"(2005DIB4J048). （2005DIB4J048）
