中国畜牧兽医2011，Vol.38Issue(9)：83-86,4.
锦鲤疱疹病毒双重PCR检测方法的研究
Detection of Koi Herpesvirus by Duplex PCR Assay
摘要
Abstract
Providing references of diagnosing koi herpesvirus (KHV) , detection of KHV was developed by duplex PCR in this study. According to genes of Sph 1-5 and thymidine kinase (TX) of KHV, special primers were designed, and the process of the duplex PCR reaction was optimized. The system described herein represent simple, sensitive and accurate detection method in which only one reaction was necessary to detect two target sequences of 292 bp and 410 bp that could be reliably used for the identification of KHV.关键词
锦鲤疱疹病毒/双重PCR/Sph Ⅰ-5/TKKey words
koi herpesvirus/duplex PCR/ Sph Ⅰ-5/TK分类
农业科技引用本文复制引用
张振国,徐立蒲,王姝,王静波,王小亮,曹欢..锦鲤疱疹病毒双重PCR检测方法的研究[J].中国畜牧兽医,2011,38(9):83-86,4.基金项目
北京市农业科技项目(20081212). （20081212）
