中国畜牧兽医2011，Vol.38Issue(9)：87-90,4.
山羊脑多头蚴线粒体nad1基因的克隆及序列分析
Cloning and Sequence Analysis of Mitochondrial nad1 Gene for Coenurus cerebralis on Goats in Hunan Province
摘要
Abstract
The objectives of the present study were to examine sequence variation in the mitochondrial NADH dehydrogen-ase subunit 1 (nadl)gene among Coenuruscerebralis isolates in Hunan province,and to reconstruct their phylogenetic relationship using nad\ sequences. The partial nadl (pnadl) vras amplified from each C. Cerebralis,and pnad1 sequences were aligned using the ClustalX 1. 81. MP and NJ trees of pnadl were constructed using the software Phylip 3. 67 version 4. 0 and Mage version 4.0,and ML tree was also constructed using Puzzle version 5. 2, sequence homology analysis was performed using the Megalign program of the software DNAStar version 5. 0. The results showed that the lengths of pnadl sequences were 430 bp. The constructed phylogenetic tree revealed that the Hunan isolates and the Taenia multiceps available in GenBank were clustered in the same clade. There is no significant variation in pnadl sequences within C. Cerebralis, while inter-species difference is obvious. It is concluded that pnadl sequences can be used as genetic marker for population genetic studies of cestodes. The results of the present study provided foundation for further studies of molecular epidemiology of C. Cerebralis,and for diagnosis of the resultant disease.关键词
脑多头蚴/线粒体DNA/nad1基因/种系发育关系Key words
C. Cerebralis/Mitochondrial DNA/nad 1 gene/phylogenetic relationship分类
生物科学引用本文复制引用
汤国祥,刘笛秋..山羊脑多头蚴线粒体nad1基因的克隆及序列分析[J].中国畜牧兽医,2011,38(9):87-90,4.基金项目
长沙科技计划一般项目(K0902144-21)资助. （K0902144-21）
