中国畜牧兽医2011，Vol.38Issue(9)：97-100,4.
水貂肠炎病毒VP2基因在昆虫杆状病毒表达系统中的表达
Expression of VP2 Gene of Mink Enteritis Virus in Baculovrius Expression System
摘要
Abstract
To obtain expression of VP2 gene of MEV in Bac-To-Bac expression system and develop MEV subunit vaccine. MEV VP2 gene was amplified by PCR method and then cloned into Pmd18-T vetcor. The VP2 gene was inserted into pFast-Bac I vector,then transferred the recombinant plasmid pFast-Bac I -VP2 into DH10 Bac to get recombinant Bacmid. Subsequently, the recombinant bacmid was transfected into Sf9 cells. The expressed recombinant protein was identified by Western blotting after three cell passages. VP2 gene of MEV was successfully cloned. The obtained recombinant VP2 protein reacted with MEV monoclonal antibody was identified by Western blotting. The VP2 gene of MEV is successfully expressed in Bac-To-Bac expression system and shows well reactivity with MEV monoclonal antibody.关键词
水貂肠炎病毒/VP2基因/病毒样粒子/杆状病毒表达系统Key words
mink enteritis virus/VP2 gene/ virus-like particles/baculovirus expression system分类
农业科技引用本文复制引用
倪佳,张蕾,柴秀丽,高晗,张海玲,赵建军,白雪,邵西群,闫喜军..水貂肠炎病毒VP2基因在昆虫杆状病毒表达系统中的表达[J].中国畜牧兽医,2011,38(9):97-100,4.基金项目
科技部科研院所社会公益研究专项"野生动物重要传染病生态学发生,监测和控制技术"(2005DIB4J048). （2005DIB4J048）
