中国畜牧兽医2011，Vol.38Issue(9)：40-43,4.
日本乙型脑炎病毒SA14-14-2株NS1-Flag蛋白在293T细胞中的表达
Eukaryotic Expression of NS1-Flag Protein of Japanese Encephalitis Virus SA14-14-2 in 293T Cells
摘要
Abstract
RT-PCR was performed on the total RNA extracted from Japanese encephalitis virus (JEV)SA14-14-2 to obtain the cDNA of NSl.the cDNA was edonuclease digestion by Hind Ⅲ/Hpa I and cloned into a vector pcDNA3. 1-Flag. The re-combinant plasmid was named plasmid pcDNA3. 1-NSl-Flag, identified correctly by DNA sequencing and restriction enzyme a-nalysis. The plasmid pcDNA3. 1-NSl-Flag was transfected into 293T cell by means of liposome and its expression was examined by immunofluorescence assay and Western blotting,the results showed that NS1 protein could express in 293T cell.关键词
日本乙型脑炎/NS1-Flag融合蛋白/真核表达/293T细胞Key words
Japanese encephalitis virus/NS1-Flag protein/eukaryotic expression/293T cell分类
生物科学引用本文复制引用
臧富玉,亓文宝,陈孝明,刘国乾,李红梅,廖明..日本乙型脑炎病毒SA14-14-2株NS1-Flag蛋白在293T细胞中的表达[J].中国畜牧兽医,2011,38(9):40-43,4.基金项目
国家自然科学基金(30800827) （30800827）
广东省科技计划项目(2009B030803049 （）
2010B020307009) （）
兽医生物技术国家重点实验室开放基金(SKLVBF201002) （SKLVBF201002）
广东省自然科学基金(06105471). （06105471）
