中国畜牧兽医2011，Vol.38Issue(9)：52-56,5.
VEGF-A、VEGF-C及PLGF基因在奶牛乳腺发育各时期的表达变化
Expression Changes of VEGF-A ,VEGF-C and PLGF Gene in Different Developmental Periods of Dairy Cow Mammol/lary Gland
摘要
Abstract
In order to study the mRNA expression changes of VEGF-A ,VEGF-C,and PLGF gene in different developmental periods of dairy cow mammol/lary gland, then lay the foundation for further research on the gene function of the cow mammol/lary gland. The SYBR Green I dye Real-time PCR method was used,and the results were compared, with gene chip differential screening results of Affymetrix company. The results showed that three genes were expressed in 11 periods of mammol/lary gland. VEGF-A gene expression during late pregnancy and lactation was higher and significantly different from other periods(P<0. 05);VEGF-C gene expression was higher during lactation; PLGF gene expression from the onset of virgin to pregnancy increases gradually to 4 months pregnancy the highest expression level, followed by gradually decreasing the expression. The results could be drawn from the above, VEGF-A and VEGF-C gene may play a role in the lactating cows, and PLGF gene might be play an important role to cow mammol/lary gland development, so get the PLGF CDS sequence by PCR amplified method, lay the test foundation for further research on the gene function of PLGF gene.关键词
VEGF基因/荧光定量PCR/乳腺上皮细胞/差异表达Key words
VEGF genes/ real-time PCR/ mammol/lary epithelial cells differential expression分类
生物科学引用本文复制引用
张岚,李庆章,边艳杰..VEGF-A、VEGF-C及PLGF基因在奶牛乳腺发育各时期的表达变化[J].中国畜牧兽医,2011,38(9):52-56,5.基金项目
国家重点基础研究发展(973)计划(2011CB100800) （973）
东北农业大学创新团队项目(CXT005-1-1/CXT005-1-2). （CXT005-1-1/CXT005-1-2）
