中国畜牧兽医2011，Vol.38Issue(9)：57-60,4.
沼泽型水牛卵泡抑素基因的真核表达研究
Eukaryotic Expression of Swamp Buffalo Follistatin
摘要
Abstract
In this study,follistatin gene was chosen as the candidate gene affecting the reproductive ability in buffalo. Re-combinant plasmid pEGFP-bFS was conducted and expressed in both buffalo fetal fibroblast (BFF) and BHK cells. pMD-bFS and pEGFP-Ni were digested with Xho Ⅰ ,Sac Ⅱ ,then eukaryotic expression vector-pEGFP-bFS was constructed. Buffalo fetal fibroblast and BHK cells were cultured with pEGFP-bFS intermixing LipofectamineTM 2000 in carbon dioxide incubator with 37 ℃ ,5% CO2 ,100% humidity. After 24 h,expression levels of recombinant vector were verified through observing directly under phase contrast fluorescence microscope, RT-PCR and Western blotting. The results indicated that bFST expressed in both buffalo fibroblast and BHK cells. This study was a very significance to produce bioactive bFS engineered plasmid and research effects of bFS on reproductive ability.关键词
沼泽型水牛/卵泡抑素/真核表达/繁殖性能Key words
swamp buffalo/follistatin/eukaryotic expression/reproductive ability分类
农业科技引用本文复制引用
邓继贤,杨秀荣,韦英明,蒋和生..沼泽型水牛卵泡抑素基因的真核表达研究[J].中国畜牧兽医,2011,38(9):57-60,4.基金项目
广西自然科学基金(桂科自0640014) （桂科自0640014）
科技创新能力与条件建设(桂科能0992028-9). （桂科能0992028-9）
