中国畜牧兽医2011，Vol.38Issue(9)：61-64,4.
猪乳铁蛋白N叶(PLF-N)在毕赤酵母中的表达及抑菌活性鉴定
Expression of Procine Lactoferrin N-lobe(PLF-N)in Pichia pastoris and Detection of its Antimicrobial Activity
摘要
Abstract
According to the aminoacid sequences of porcine lactoferrin N-lobe(PLF-N)and partiality codon of Pichia pastoris , the porcine lactoferrin N-lobe gene was designed and synthesized. The modified porcine lactoferrin N-lobe gene was cloned into the pGAPZo-A vector to construct the recombinant expression vector pGAPZα-A-PLF-N. The Avr Ⅱ linearized plasmid pGAPZo-A -PLF-N was transformed into Pichia pastors SMD1168 by electroporation. The transformants were identified by PCR,screened with Zeocin.and multiply-copy colonies were harvested,in which PLF-N gene was verified to inserted into yeast chromosome stably. Under the control of the promoter GAP,the PLF-N recombinant protein with a molecular weight of 38 ku was expressed in supernatant. Up to 364 μg/mL PLF-N protein was produced in culture medium. Agrose diffusion assay showed that PLF-N had broad-spectrum antibacterial abilities not only to gram-negative bacteria but also to gram-positive bacteria and with MIC of 12. 5,25 μg/mL against E. Coli DH5α and Staphylococcus aureus, respectively.关键词
猪乳铁蛋白N叶/毕赤酵母/分泌表达/抑菌活性Key words
porcine lactoferrin N-lobe/ Pichia pastors/ secretion expression antibacterial activity分类
生物科学引用本文复制引用
何俊,刘德辉,林云雄,张春红,黄毓茂..猪乳铁蛋白N叶(PLF-N)在毕赤酵母中的表达及抑菌活性鉴定[J].中国畜牧兽医,2011,38(9):61-64,4.基金项目
广东省现代农业生猪产业体系(F10021). （F10021）
