中国畜牧兽医2011，Vol.38Issue(9)：175-177,3.
一株新分离犬细小病毒灭活疫苗的制备及免疫效果研究
Preparation and Immunity Effect Assessment of an Inactivated Vaccine against Canine Parvovirus
孙相鑫 1刘晔 1杨洋 1张守峰 1扈荣良1
作者信息
- 1. 中国人民解放军军事医学科学院军事兽医研究所流行病与病毒病学研究室,吉林长春 130122
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摘要
Abstract
The canine parvovirus strain JL0911 was isolated by our lab with some mutations in the main epitopes compared to the classical CPV strains isolated in China. This canine parvovirus strain could not be classified into CPV-2a, CPV-2b or CPV-2c. The aim of this study is to develop an inactivated vaccine with the JL0911 canine parvovirus strain. The canine parvovirus JL0911 was prepared in F81 cells and inactivated by formalin. A kind of nanometer adjuvant prepared by our lab was mixed with the inactivated canine parvovirus with the proportion of 1:4. Dogs were vaccinated with 1. 5 mL of this vaccine and the neutralizing antibody was detected by virus neutralization test in the F81 cell line. The results showed that the neutralizing antibody titers of the experimental dogs were elevated significantly after vaccinated 14 days by the inactivated vaccination. The most significant elevation was 29. This indicated that the canine parvovirus strain JL0911 was potential for developing inactivated vaccine with high performance.关键词
犬细小病毒/中和试验/灭活疫苗/免疫试验Key words
canine parvovirus/ neutralization test/inactivated vaccine/ immunity test分类
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孙相鑫,刘晔,杨洋,张守峰,扈荣良..一株新分离犬细小病毒灭活疫苗的制备及免疫效果研究[J].中国畜牧兽医,2011,38(9):175-177,3.
