中国畜牧兽医2011，Vol.38Issue(9)：155-159,5.
副猪嗜血杆菌的分离鉴定及药敏试验
Isolation, Identification and Antibiotic Susceptibility Test of Haemophilus parasuis
摘要
Abstract
The purpose of this study is to determine if there is Glasser's disease in Heilongjiang,which provide a theory basis for the prevention and control of Glasser's disease. The dead piglets with polyserositis and arthritis were collected and the bacterium was isolated from the samples. Then the physical and chemical properties of isolated strain were identified, its 16S rRNA gene was amplified by PCR,and this DNA fragment was subsequently cloned and sequenced. The sequenced results were analyzed by BLAST in GenBank. The homology and evolution relations analysis of the similar sequences were done by DNAS-tar. The drug sensitivity testing was done by isolated strain. The results indicated that a pantomorphic NAD dependent strain was isolated, the results of the physical and chemical properties and PCR showed that was Haemophilus parasuis. The phyletic evolution analysis results of the isolated strain 16S rRNA gene showed that it was in same subgroup with the Haemophilus parasuis in China,Japan and American,however that was in single subgroup in Spain; drug sensitivity testing showed that it was hypersensitive to spectinomycin and cephradine.关键词
副猪嗜血杆菌/分离/鉴定/药敏试验Key words
Haemophilus parasuis/ isolation/ idendifications antibiotic susceptibility test分类
农业科技引用本文复制引用
周玉龙,夏伟,陈永松,耿晶,刘辉,詹天龙,李白,侯喜林,朴范泽..副猪嗜血杆菌的分离鉴定及药敏试验[J].中国畜牧兽医,2011,38(9):155-159,5.基金项目
黑龙江省科技厅重点推广项目(TC10B0701). （TC10B0701）
