中山大学学报（自然科学版）2011，Vol.50Issue(5)：87-92,6.
香蕉乙二醛酶基因MaGLO14的克隆及在非生物胁迫下的功能鉴定
Isolation and Functional Identification of Banana Glyoxalase Gene (MaGLO14) Under Various Abiotic Stresses
摘要
Abstract
In order to identify the function of glyoxalase gene in banana, a full length cDNA of glyox-alase gene named MaGL014 had been isolated based on the partial sequence cloned by SSH previously from postharvest banana fruit. MaGLO14 contained a 1 074 bp ORF and encoded 358 amino acids. NCBI Blastx results showed that the deduced amino acid of MaGL014 shared identities of 82% , 83% , 82% , 80%, 82% with Picea sitchensis ( ABK22263 ), Vitis vinifera ( CBI23235 ), Citrus paradisi (CAB09799) , Gossypium hirsutum (ACJ11750), and Ricinus communis (EEF43857), respectively. Tissue-specific expression results indicated that MaGLO14 constitutively expressed in banana root, stem, leaf, flower and fruit. The expression levels were up-regulated under NaCl, chilling and ethepon treatments. The expression levels were down-regulated under drought and waterlogging treatments. And the MaGL014 transgenic tobacco leaves showed enhanced salt-tolerance.关键词
香蕉/乙二醛酶/克隆/表达分析/胁迫/转基因Key words
banana/glyoxalase/clone/expression analysis/stress/gene transformation分类
生物学引用本文复制引用
刘菊华,邓成菊,金志强,谢学立,贾彩红,张建斌,徐碧玉..香蕉乙二醛酶基因MaGLO14的克隆及在非生物胁迫下的功能鉴定[J].中山大学学报（自然科学版）,2011,50(5):87-92,6.基金项目
现代农业产业技术体系建设专项资金资助项目(CARS-32) （CARS-32）
热带生物技术研究所中央级公益性科研院所基本科研业务费专项资金资助项目(ITBB110202) （ITBB110202）
