中国畜牧兽医2011，Vol.38Issue(9)：190-193,4.
H1N1猪流感病毒血凝素蛋白抗原表位的筛选及其抗原性分析
Epitope Screening and Antigenicity Analyzing of Hemagglutinin of H1N1 Swine Influenza Virus
摘要
Abstract
In an attempt to isolate and identify the.epitopes on hemagglutinin (HA) of H1N1 swine influenza virus and to test their antigenicity,polyclonal mouse antisera IgG against recombinant HA was prepared to screen a 12-mer random peptide library. After three rounds of biopanning a phage enrichment was resulted. Forty of the 47 phage clones selected randomly showed to be positive by phage-ELISA and shared 3 amino acid sequences. The result of Western blotting showed that the 3 peptides could bind specially with the polyclonal mouse antisera IgG against H1N1 swine influenza virus indicated that the 3 peptides was antigenicity. In this paper,three antigenic epitopes on HA were obtained and established the foundations for the further research of vaccine and diagnoses of H1N1 swine influenza virus.关键词
噬菌体肽库/抗原表位/猪流感病毒/血凝素蛋白Key words
phage displayed peptide library/antigen epitopesswine influenza virus/hemagglutinin分类
农业科技引用本文复制引用
唐连飞,易征璇,朱中武,朱金国,陈盼,孟芳,禹思宇,欧阳振宇,姜金林..H1N1猪流感病毒血凝素蛋白抗原表位的筛选及其抗原性分析[J].中国畜牧兽医,2011,38(9):190-193,4.基金项目
国家质量监督检验检疫总局科技项目(20071K009). （20071K009）
