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鹅大肠杆菌的分离鉴定及药敏试验

严玉霖 刘小克 雷华 杨尚斌 祝常芬 蒋欢 杨素红 何诸林 李丽芝 钟义广

中国畜牧兽医2011,Vol.38Issue(10):160-162,3.
中国畜牧兽医2011,Vol.38Issue(10):160-162,3.

鹅大肠杆菌的分离鉴定及药敏试验

Separation,Identification and Drug Sensitive Test of Escherichia coli from Goose

严玉霖 1刘小克 2雷华 2杨尚斌 3祝常芬 1蒋欢 1杨素红 2何诸林 2李丽芝 2钟义广2

作者信息

  • 1. 云南农业大学动物科学技术学院,云南昆明 650201
  • 2. 云南云鹅牧业有限公司,云南昆明 650300
  • 3. 云南省大理农业学校,云南大理671003
  • 折叠

摘要

Abstract

In order to direct the treatment of goose E. Coli disease and establish the foundation of preparation for the goose E. Coli inactivated vaccine. The suspected cases of the goose E. Coli disease were taken and separated and cultivated in MacConkey medium. The bacteria strains were obtained,and identificated by morphological assay,biochemistry assay,serotype assay, and animal experiments. Moreover, the identified E. Coli were carried out by drug susceptibility test. The results showed that the strains of E. Coli were pink,moist,smooth and neat in MaConkey medium,which were small,negative of Gram's staining, ends obtuse and many individual exists. The isolated bacteria were identified as E.coli,which animal experiment showed that the goose E.coli had higher infection rate and fatality rate. The 15 strains were identified by serotype assay: 4 O35 strains,3 O78 strains,3 O26 strains, 2 O72 strains, 1 O109 strains, 1 O141 strain, 1 O2 strain. In vitro antibacterial experiment results showed that Doxycycline and Amoxicillin against the above E. Coli had strong inhibited effect. The research provide the theoretical foundation and significant applical value for efficientively preventing and curing goose E. Coli disease.

关键词

/大肠杆菌/分离鉴定/药敏试验

Key words

goose/E. Coli/isolation and identification/drug sensitive test

分类

农业科技

引用本文复制引用

严玉霖,刘小克,雷华,杨尚斌,祝常芬,蒋欢,杨素红,何诸林,李丽芝,钟义广..鹅大肠杆菌的分离鉴定及药敏试验[J].中国畜牧兽医,2011,38(10):160-162,3.

基金项目

云南省高端科技人才引进计划项目(2009C1125) (2009C1125)

清源朗德鹅技术中心建设及标准化养殖技术示范(08N060401). (08N060401)

中国畜牧兽医

OA北大核心CSTPCD

1671-7236

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