中国畜牧兽医2011，Vol.38Issue(10)：214-217,4.
猪戊型肝炎病毒ORF2部分片段原核表达载体的构建
The Construction of Prokaryotic Expression Vector of Swine Hepatitis E Virus ORF2 Fragment
摘要
Abstract
The test used the Protean software to analyze the potential antigenic sites of ORF2 of HEV swCH189 strain,and selected 381aa to 623aa segment as the expression of target.the cp239 fragment was amplified from the full-length ORF2 plas-mid by PCR,after purification, connection, and so on. The prokaryotic expression vector pET32a(+ )-cp239 was successfully constructed. This work laid the foundation for the next step of the prokaryotic expression and analysis of biological activity.关键词
猪/戊型肝炎病毒/ORF2/表达载体构建Key words
swine/hepatitis E virus/ORF2/construction of prokaryotic expression vector分类
生物科学引用本文复制引用
郝宝成,兰喜,胡永浩,柳纪省,梁剑平..猪戊型肝炎病毒ORF2部分片段原核表达载体的构建[J].中国畜牧兽医,2011,38(10):214-217,4.基金项目
国家"十一五"辩技支撑计划项目(2006BAD04A05-05) （2006BAD04A05-05）
中央级公益性科研院所基本科研业务费专项资金(BRF090402). （BRF090402）
