传感技术学报2011，Vol.24Issue(9)：1242-1247,6.DOI:10.3969/j.issn.1004-1699.2011.09.003
CdTe/CdS量子点在Nafion/羟基磷灰石膜上的电化学发光及其应用于肌红蛋白的高灵敏检测
Electrochemiluminescence of CdTe/CdS Quantum Dots Immobilized on Nation/HAp Hybrid Film and Its Application to Highly Selective Detection of Myoglobin
摘要
Abstract
CdTe/CdS QDs were immobilized on glassy carbon electrode ( GCE ) using hydroxyapatite ( Hap ) and Nation. Compared with the ECL intensity of QDs dispersed in aqueous solution with the bare GCE, the chemically modified electrode could greatly enhance the ECL intensity of CdTe/CdS QDs in the presence of tri-n-propylamine (TPrA) as coreactant. A new ECL method has been developed for the determination of myoglobin ( Mb) based on its quenching effect on the ECL intensity of CdTe/CdS quantum dots in pH 6.5 phosphate buffer solution (PBS). The results demonstrated that myoglobin could effectively quench the QDs emission based on the possible quenching mechanisms. Under the optimal conditions, the ECL intensity was decreased with the increase of Mb concentration in the range of 0.59 nmol/L ~ 10.59 nmol/L with the detection limit of 0.12 nmol/L.关键词
CdTe/CdS量子点/电化学发光/羟基磷灰石/三丙胺/肌红蛋白Key words
CdTe/CdS quantum dots/ electrochemiluminescence/ Hydroxyapatite/ Tri-n-propylamine/ Myoglobin分类
信息技术与安全科学引用本文复制引用
王国涛,贾丽萍,赵倩,陶绪泉,贾文丽,崔慧,王怀生..CdTe/CdS量子点在Nafion/羟基磷灰石膜上的电化学发光及其应用于肌红蛋白的高灵敏检测[J].传感技术学报,2011,24(9):1242-1247,6.基金项目
国家自然科学基金项目(20775031,21075059) （20775031,21075059）
