山东医药2011，Vol.51Issue(41)：7-9,3.
左旋丁基苯酞对Aβ1-42诱导的体外原代培养神经元损伤的影响
Neuroprotective effects of the L-3-N-butylphthalide on the damage of cultured primitive rat basal forebrain and hippocampus neurons induced by Aβ1-42
摘要
Abstract
Objective To investigate the neuroprotective effects of L-3-N-butylphthaIide ( L-NBP) on the damage of cultured primitive rat basal forebrain and hippocampus cholinergic neurons induced by beta-amyloid peptides (Aβ1-42). Methods Primitive rat basal forebrain and hippocampus neurons were cultured and evaluated. Neurocyte trauma model was established by Aβ1-42(2 μmmol/L). Neurocytes were divided into control group, Aβ1-42 model group, Aβ1-42 + 1-NBP lower, middle, higher group(0.1 、1、10 μnmol/L) . Observing cells morphologic change in inverted microscope, sand NF-Kb, Nnos levels were detected by ELISA. Results Being exposed to Aβ1-42 with 48 h, neurons morphologic had significantly changed, the expression of NF-Kb in the cultured neurons was increased significantly and the expression of Nnos was decreased significantly compared with the controls ( P < 0. 05 ). Neurons morphologic had significantly changed with different doses of 1-NBP, and 1-NBP could inhibit NF-Kb activation, increase the expressions of Nnos ( P < 0.05 ). Conclusion The neuroprotective effects of 1-NBP on cultured neurons induced by Aβ1-42 result from adjusting the expressions of NF-Kb and Nnos.关键词
左旋丁基苯酞/β淀粉样蛋白/基底前脑/海马/NF-kB/nNOS/大鼠Key words
L-3-N-butylphthalide/ beta-amyloid peptides/ basal forebain/ hippocampus/ NF-Kb/ Nnos分类
医药卫生引用本文复制引用
王瑞霞,蒋亮亮,马国诏,付庆喜,李甲龙,闫鹏,张镛..左旋丁基苯酞对Aβ1-42诱导的体外原代培养神经元损伤的影响[J].山东医药,2011,51(41):7-9,3.基金项目
山东省医药卫生科技发展计划项目(2007HZ06). （2007HZ06）
