基础医学与临床2011，Vol.31Issue(12)：1314-1319,6.
MnSOD过量表达与BSO对食管癌细胞系的增敏效应
Radiosensitization effect of MnSOD and BSO on esophageal cancer cells by overexpression of MnSOD and BSO
摘要
Abstract
Objective To explore effect of manganese superoxide dismutase ( MnSOD) overexpression on rediosen-sitivity in the esophageal cancer cell line in the presence of buthionine sulfoximine (BSO). Methods Overexpression of MnSOD Cdna by lentivirus to get stable transfected TE-1 cell lines of MnSOD overexpression { TE-1 Mm ). Reverse transcription polymerase chine reaction (RT-PCR) , Western blot and flow cytometry were then used to detect MnSOD overexpression that might be found on cell proliferation, cell apoptosis, cell radiosensitivity, eactive . Oxygen species (ROS) fluorescence index. Results RT-PCR and Western blot showed that TE-1 cells transfected with MnSOD contained the target genes in overexpression. Plating efficiency was reduced in the MnSOD overexpression transfected cell line and decreased the plating efficiency. While the TE-1 cell had a higher plating efficiency. Furthermore, apparent apoptosis of TE-1 Mm cell induced by transfected MnSOD overexpression was demonstrated by Annexin V-FITC/PI staining compared to TE-1 cell. Moreover, in multitarget single-hit model-fitting survival curves, the values of DO, Dq, N, and SER in TE-1 Mm cell lines were less than in TE-1 cell. FCM results indicated that TE-1 Mm of reactive oxygen species ( ROS) fluorescence index was less than that in TE-1 cell. Western blot demonstrated that TE-1 Mm had a higher MnSOD protein expression than those of TE-1 treated with BSO and radiation. Conclusions MnSOD overexpression can increase the radiosensitivity of esophageal cancer cells by inhibiting cell proliferation, inducing cell apoptosis and decreasing ROS concention in combination with BSO.关键词
锰超氧化物歧化酶/丁胱亚磺酰亚胺/食管肿瘤/放射增敏Key words
manganese superoxide dismutase/ buthionine sulfoximine/ esophageal carcinoma/ radiosensitivity分类
医药卫生引用本文复制引用
孙国贵,胡万宁,于秀荣,杨从容,王雅棣..MnSOD过量表达与BSO对食管癌细胞系的增敏效应[J].基础医学与临床,2011,31(12):1314-1319,6.基金项目
国家自然科学基金(30540005) （30540005）
国家人事部高层次留学归国人员资助国人厅发[2005] 118号 （）
教育部留学归国人员启动基金教外司[2008]101号 （）
