基础医学与临床2011，Vol.31Issue(12)：1376-1382,7.
CCC-HPF-2细胞基因组DNA可作为STR分型标准物质
Genomic DNA of CCC-HPF-2 cells may function as a reference standard for STR profiling
杨振丽 1卞晓翠 1刘玉琴 1王春景 1苏小玲 1顾蓓 1张宏1
作者信息
- 1. 中国医学科学院基础医学研究所细胞资源中心,北京100005
- 折叠
摘要
Abstract
Objective To establish and identify a cell line originated from Chinese as reference standards for STR profiling and to establish reference STR-cell bank. Methods The cell morphology of various passages were observed under microscope. The expression of vimentin and CK was detected immuno-cytochemically. The karyotype was analysed. Contamination of mycoplasma and the species origination of the cell line were checked by PCR. The stability and balance of STR profiling were analysed. Results The cell was fibroblast. The expression of vimentin was positive, CK is negative. No contamination of mycoplasma was found. All the 15 STR loci were well amplified and in good balance. STR profiling was completely identical among various passages. Conclusions The cell was originated from human. Under 30 passage DNA from these cells can be used as reference standards for STR profiling.关键词
短串联重复序列/STR/细胞库/标准物质Key words
STR Profiling/ STR/ Cell banking/ reference standards分类
医药卫生引用本文复制引用
杨振丽,卞晓翠,刘玉琴,王春景,苏小玲,顾蓓,张宏..CCC-HPF-2细胞基因组DNA可作为STR分型标准物质[J].基础医学与临床,2011,31(12):1376-1382,7.
