福建农林大学学报（自然科学版）2011，Vol.40Issue(5)：461-466,6.
圆果黄麻成熟叶片总DNA提取及SRAP扩增体系的建立与优化
Extraction of genomic DNA from adult jute leaves, and establishment and optimization of SRAP reaction system
摘要
Abstract
Genomic DNA from jute (Corchorus capsularis L. ) extracted with modified CTAB method was tested for its concentration and quality by agarose gel electrophoresis and concentration detection. An optimum SRAP-PCR system was established based on optimizing the key factors of SRAP - PCR reaction system in jute. The results showed that the DNA isolated with modified CTAB method was in good quality, pure and integral. Z)260 nm/D280 nm value of the DNA was 1.7-1.9 using ultraviolet spectrophotometer determination. There was no degradation appeared and the proteins, phenols, etc. Were eliminated completely. The optimum SRAP-PCR reaction system could amplify high levels of polymorphism, had good repeatability and clear band pattern. SRAP system (total volume of 25 μL) was established as follows; Taq DNA polymerase 1.75 U, Mg2+ 3.0 mmol · L-1, dNTPs 0.16 mmol · L-1, forward primer 0.51 μmol · L-1, reverse primer 0.51 μmol · L-1, template DNA 80 -120 ng. It was showed that the optimized system in this study can be applied in the SRAP analysis for jute.关键词
圆果黄麻/DNA提取/SRAP反应体系Key words
jute (Corchorus capsularis L. ) /DNA extraction/sequence-related amplified polymorphism (SRAP)分类
农业科技引用本文复制引用
陈燕萍,陈美霞,徐建堂,陈晖,陶爱芬,祁建民..圆果黄麻成熟叶片总DNA提取及SRAP扩增体系的建立与优化[J].福建农林大学学报（自然科学版）,2011,40(5):461-466,6.基金项目
国家自然科学基金(30571188)资助项目 （30571188）
国家麻类产业技术体系建设项目(nycytx-19-E05). （nycytx-19-E05）
