西华大学学报（自然科学版）2011，Vol.30Issue(5)：96-99,4.
聚乙二醇沉淀猪胰蛋白酶的研究
Precipitation of Porcine Trypsin by Polyethyleneglycol
摘要
Abstract
To study the condition for extractaction of porcine trypsin by PEG (polyethyleneglycol) ,the effect of molecular weight and concentration of PEG, pH and extraction time on trypsin activity was investigated by both single factor experiment and orthogonal factor experiment. The optimum technology was determined by experiment as follows; 0. 25% of PEG 6000 with pH equal to 7. 5 and temperature of 4 ℃ while the extraction time was 3 h. Under the optimum conditions, the activity of the trypsin reached above 1600 u/ mL, higher than that of traditional ammonium sulphate precipitation. This method is effective to isolate trypsin from porcine pancreas.关键词
猪胰蛋白酶/聚乙二醇(PEG)/沉淀Key words
porcine trypsin/polyethylene glycol/precipitaion分类
化学化工引用本文复制引用
余武英,赵金梅,肖青虎,王维香..聚乙二醇沉淀猪胰蛋白酶的研究[J].西华大学学报（自然科学版）,2011,30(5):96-99,4.基金项目
西华大学重点课程(《酶工程》)项目 （《酶工程》）
